Journal: Italian Journal of Animal Science

Article Title: Pterostilbene attenuates oxidative stress induced by hydrogen peroxide in MAC-T cells through activating PINK1/Parkin-mediated mitophagy

doi: 10.1080/1828051x.2024.2353853

Figure Lengend Snippet: Figure 3. Pterostilbene (PTE) attenuated H2O2-triggered oxidative damage to MAC-T cells. Pre-treatment with PTE (10 lM) was performed for 12 h, followed by H2O2 (600 lM) treatment of MAC-T cells for 24 h. (a) Representative Western blot bands for HO-1, SOD1 and GPX4 proteins. (b) Quantitation of HO-1 protein level in MAC-T cells. (c) Quantitation of SOD1 protein level in MAC-T cells. (d) Quantitation of GPX4 protein level in MAC-T. (e) Quantitation of NRF2 protein level in MAC-T data are present as mean ± SEM (n ¼ 3), p < 0.01 versus control group, ##p < 0.01 versus H2O2 group.

Article Snippet: Subsequently, the membranes were blocked with 3% defatted milk powder (P0216, Beyotime, China) for 2 h at room temperature and incubated with primary antibodies at 4 �C overnight against HO-1 (27282-1-AP, Proteintech, China), SOD1 (10269-1-AP, Proteintech), GPX4 (14432- 1-AP, Proteintech), Parkin (14060-1-AP, Proteintech), PINK1 (23274-1-AP, Proteintech), beclin-1 (11306-1-AP, Proteintech) and GAPDH (60004-1-Ig, Proteintech).

Techniques: Western Blot, Quantitation Assay, Control

Journal: Lipids in Health and Disease

Article Title: Isocaloric intake of a high-fat diet modifies adiposity and lipid handling in a sex dependent manner in rats

doi: 10.1186/1476-511X-10-52

Figure Lengend Snippet: Levels of proteins involved in adipose tissue lipid uptake and mobilization

Article Snippet: Rabbit antibody against PKA and goat antibodies against α2- and β3-AR were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Control